The countess II FL is the major component of this equipment and is the core module that enables highly accurate, reproducible and high throughput cell counting. The Countess II reuse slide enables the same slide to be used for an infinite number of experiments and reduces consumable costs associated with individual slides. However, this equipment allows options for users who need individual slides at their own expense. The two EVOS LED Cubes are removable components that allow quantification of fluorescent cells. We have chosen green fluorescent protein and red fluorescent protein as these are the major fluorophores used. However, these can be switched out for other detectors in the future, making it future proof and adaptable to future research needs.

Broad research use of equipment: The Invitrogen Countess II FL is an Automated Cell Counter with a reusable slide and fluorescence capabilities that can accurately count cells, monitor fluorescent protein expression, and measure cell viability with applications to both basic and clinical research projects requiring cell quantification.

It is suitable for the quantification of both model and human cells. The reusable slide makes it highly affordable by significantly reducing consumable costs, which is ideal for EMCRs. The accuracy of the Countess II FL eliminates the subjectivity of manual cell counting and minimizes user-to-user variability enabling highly reproducible results. It also eliminates the need for manual microscope time for cell counting, which has implications for staff and student health and safety by reducing fatigue and exposure to lasers. The speed of the Countess II FL allows quantification of both live and dead cells for determining cell viability as well average cell size in as little as 10 seconds, which will significantly increase throughput and productivity. The fluorescent cubes are interchangeable and can be customized to suit individual user needs. We will purchase the two mainstay fluorescent channels (e.g. green and red fluorescent protein) with this grant, but additional cubes may be purchased by individual users/teams as required. These fluorescent capabilities will have broad experimental implications that are cost effective and assist in reducing the burden on the HMRI Core Flow Cytometry facility for some applications.


Dr Malcolm Starkey, Dr Adam Collison, Dr Hock Tay, Dr Aniruddh Deshpande, Dr Gang Liu & Dr Jemma Mayall

Project type 
Equipment Grant
Year of funding